Gene unlocking, assembly, passive effect

Regarding plasmids:

Assembled genes are divided into the main genome region and plasmid region. The plasmid area allows you to create or obtain plasmids. There is no upper limit on the number of plasmids. Each plasmid is also divided into Active genes and Potential genes.

Plasmid creation:

Create a new blank plasmid, each with a basic cell replication phosphate cost (From ORI). Compared to the main genome, the MP consumption of genes entering the plasmid genome from unassembled genes is less, and the MP consumption of gene deletion and movement between Active genes and Potential genes is also less.

Transferring the second gene into the plasmid also consumes less MP.

A well-developed nucleus should result in less consumption of gene movement in the main genome than in plasmids.

Plasmid management:

Allow genes to be identical between plasmids and the main genome, as well as between plasmids.By consuming MP, you can copy genes from the main genome or other plasmids into one plasmid. The deletion of entire plasmids do not consume MP.

Allowing the consumption of MP to merge plasmids into the main genome, Keep one duplicate gene, the merged plasmid will not disappear directly and needs to be manually deleted.
Two plasmids also can be merged into one plasmid. It is necessary to select a main plasmid to be replaced, and the merged plasmid will not disappear.

Allow the selection of plasmid consumption MP for seal, and the number of sealed plasmids is limited. The sealed plasmid will not have an impact on your genome’s passive and phosphate replication costs, and can consume MP to unlock.

Plasmid transfer:

In addition to this method of obtaining a single gene, plasmids allow you to obtain the entire plasmid. You have the opportunity to directly obtain plasmids from other organisms and consume MP to add them to your own plasmid group.

Plasmid incompatibility(doubtful ):

It is generally believed that if the ORI carried by two plasmids belong to the same type, they will use the same cell replication mechanism. Plasmids with the same ori are incompatible with each other.

This situation generally comes from homologous plasmids. Require homologous plasmids to choose a reservation (which can extract genes from it) or merge.


About ORI:
ORI is the site at which DNA replication begins(Plasmids only). ORI actually affects the replication ability of plasmids, but it is also regulated by specific cells. I don’t have any ideas for this part yet. Perhaps it can be considered from duplicate genes? But this should be too complicated.

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